Hyelin Ji

• Team - Biopharmaceutical pharmacology and toxicology team
• Project - Adeno associated virus (AAV) drugs for neuropathic pain
• Specific job

1) Set-up the disease animal models

2) Set-up the experimental methods

3) Efficacy test with new drugs

4) Plan and regulate the bio-distribution study

5) Plan and regulate the toxicity study

6) Mode of action study

7) Preparing the IND application

8) Apply the patent and defend the Officer Action (OA)

• Research paper - Kim D, Kim KR, Kwon Y, Kim M, Kim MJ, Sim Y, Ji H, Park JJ, Cho JH, Choi H, Kim S. AAV-Mediated Combination Gene Therapy for Neuropathic Pain: GAD65, GDNF, and IL-10. Mol Ther Methods Clin Dev. 2020 Jun 25;18:473-483. doi: 10.1016/j.omtm.2020.06.018. PMID: 32728596; PMCID: PMC7378317.
• Research skill

1) Animal disease model

- Spared nerve injury (SNI)

- Streptozocin induced diabetic model

- 6-OHDA induced parkinson's disease

2) Injection

- Transforaminal epidural

- Interlaminar epidural

- Intrathecal

- Intraperitoneal

- Intravenous

- Intramuscular

- Subcutaneous

- Stereotaxic

3) Analysis

- von Frey filament

- Apomorphine induced rotation

- Rota-rode

- Tissue harvest (Dorsal root ganglion, spinal cord, other tissues...)

- Immunohistochemistry (sectioning, staining)

- Imaging (Confocal microscope, analysis)

- Jugular vein blood collection

• Desk work

1) Experiment planning

- Writing and managing IACUC document

- Non-GLP and GLP study for BD and tox

2) Setting the standard operating procedure (SOP)

- Transforaminal epidural injection

- Intrathecal injection

- von Frey filament test

- Histology (perfusion, sectioning, staining, imaging)

- Tissue harvest for BD and tox

3) Report the results

- Efficacy (dose dependent, sex differences, etc...)

- Mode of action (Anti-apoptosis, anti-inflammation, etc...)

- non-GLP BD and tox

4) Preparing IND (investigational new drug)

- Regulate the timeline

- Review the research reports (M3, M4, etc...)

5) ETC

- Annual plan of pipeline

- Annual budget plan

- Applying the patent and depend the OA (officer action)

• Neuroscience laboratory (homepage - www.http://neurolab.yonsei.ac.kr/)
• Projects

1) Electroencephalogram (EEG) analysis of sleeping mouse after gene knock out of knock down

2) Finding neural pathway of thalamocortical brain network using retrograde beads

3) Finding the most effective route of administration of new drugs

• Specific technique

1) Stereotaxic injection

2) Intraperitoneal injection

3) intravenous injection

4) intramuscular injection

5) perfusion

6) brain harvest

7) EEG surgery

8) Immunohistochemistry

9) imaging (confocal microscope)

10) EEG analysis using MATLAB

• ET Cetra (ETC)

1) lab safety (LMO, goverment laws...)

2) writing and administrate IACUC documents