KIM TAEHYUN
인천광역시 검단구
Summary
Recent B.S. graduate with research experience in yeast metabolic engineering at the Microbial Metabolic Engineering Lab (Prof. Won-Heong Lee)
Overview
1
1
Language
1
1
Certification
1
1
year of professional experience
Work History
Undergraduate Researcher
Microbial Metabolic Engineering Lab
Gwangju, South korea
02.2025 - 08.2026
- Conducted research on yeast metabolic engineering projects focused on mixed-sugar utilization, glucose repression, and alternative carbon-source metabolism.
- Designed gRNAs for CRISPR/Cas9-mediated gene deletion and analyzed mixed-sugar fermentation profiles to investigate candidate genes involved in glucose repression alleviation in Saccharomyces cerevisiae.
- Engineered lactose-utilizing Saccharomyces cerevisiae strains through PCR, molecular cloning, plasmid construction, and yeast transformation of lactose-utilization genes.
- Conducted flask-scale fermentation experiments using Saccharomyces cerevisiae and Kluyveromyces spp. under various single- and mixed-sugar conditions, with time course sampling and HPLC-based analysis of sugar consumption and fermentation metabolite profiles.
- Applied adaptive laboratory evolution and phenotypic screening to select Kluyveromyces lactis strains with improved xylose utilization.
- Attempted GFP-fusion protein analysis of MTH1 and mutant MTH1, including yeast protein extraction, BCA assay, SDS-PAGE, and anti-GFP Western blotting.
- Addressed unexpected experimental results across research projects through repeated trials, comparison of cultivation conditions, and investigation of possible causes based on published studies.
Education
Bachelor of Science - Bioenergy Engiceering
Chonnam National University
Gwangju, 29 2020 - 2026
Skills
Yeast & E coli cultivation
Flask-scale yeast fermentation
HPLC analysis
Molecular cloning & yeast / E coli transformation
CRISPR/Cas9 genome editing
ALE & phenotypic screening
Protein analysis
Languages
English
Intermediate
B1
Certification
- 바이오화학제품제조기사
Timeline
Undergraduate Researcher
Microbial Metabolic Engineering Lab
02.2025 - 08.2026
Bachelor of Science - Bioenergy Engiceering
Chonnam National University
2020 - 2026