Park Siyeong

Project 1: Development of High-Sensitivity Real-Time Human DNA Sensors for Forensic Applications

• Extracted human gDNA from diverse forensic casework samples (swabs, fabrics, blood) utilizing silica-membrane technology (QIAamp DNA Micro Kit, Qiagen).
• Quantified DNA and verified sample purity via Real-time PCR (BIO-RAD), rigorously maintaining high-precision standard curves ($R^2 \ge 0.998$).
• Conducted STR analysis (Thermo Fisher) to assess the degree of DNA degradation and evaluate the viability of individual identification.

Project 2: Field Application of Novel Fluorescent Reagents for Latent Fingerprint Detection on Adhesive Tapes

• Established and optimized the overall cell culture environment, maintaining human cancer cell lines (SK-MEL-5, A549).
• Evaluated the cytotoxicity of test compounds using WST assays to ensure reagent safety.
• Managed the procurement of research materials and drafted operational budget proposals for the project.

Project 3: Advanced Gas Analysis Techniques for Toxic Substances and Postmortem Investigations

• Processed samples using GC-MSD (Agilent) according to standard operating procedures (SOPs), managing rigorous washing protocols and the operational status of gas collection tubes.
• Managed the comprehensive project budget, overseeing expenditure planning and resource allocation.

Project 1: Development of High-Sensitivity Human DNA Detection Sensors

• Cultured diverse human cancer cell lines (HepG2, MDA-MB-231, MCF-7) and established reference values for DNA extraction yield based on cell count using spectrophotometry.
• Conducted comparative assays to develop a novel DNA extraction reagent demonstrating a higher yield than the standard QIAamp DNA Micro Kit.

Project 2: Development of Cultured Seafood Production Technology

(Role: Team Leader)

• Developed and optimized primary culture protocols for various marine organisms, including shrimp, oyster, and pen shell.
• Performed qPCR and gel electrophoresis to analyze and verify the expression of specific genetic markers.
• Managed comprehensive experimental data for cell culture and molecular assays, and prepared technical reports and presentations for the Principal Investigator (PI).

Project 3: Evaluation of Cell Engraftment on Experimental Scaffolds

(Role: Team Leader)

• Led the optimization and comparative analysis of cell engraftment rates across four distinct types of novel scaffolds utilizing the MACK1 (mackerel) cell line.
• Performed live/dead cell viability assays using Propidium Iodide (PI) and Calcein AM staining, and visualized the results via confocal fluorescence microscopy.