Experienced and knowledgeable in nanomedicine with a strong foundation in scientific research methodologies and a keen interest in applying them to develop innovative solutions in healthcare. Proven expertise in nanomaterial synthesis, characterization techniques, Immunotherapy, bioinformatics, cheminformatics, and gene and protein engineering. Adept at designing and conducting experiments, analyzing data, and interpreting results. Demonstrated skills in scientific writing, critical thinking, and problem-solving. Collaborative team members actively contribute to interdisciplinary projects and possess a strong commitment to advancing scientific knowledge in the field of nanomedicine.
Microscopy techniques- Cell and tissue-based assays with Inverted light microscopy, and fluorescence microscopy
Immunological techniques- Cell induction isolation, ELISA, fluorometric assay, isolation of primary immune system cells from animals, cell culture, etc
Histopathology techniques- Tissue fixation, Microtome, staining, Immuno-histological staining, etc
Animal cell culture- Maintain, subculture, freezing, thawing, revival, cell toxicity and specific induction of cell lines cryogenic storage, cell-based assays, and organ-based primary cell culture
Flow cytometry techniques- Apoptosis, Protein expression, cell population after induction and treatment Differential fluorescence staining procedures
Animal handling- Mouse and rat handling, Drug administration via intragastric incubation subcutaneous intraperitoneal and tail vein intravenous injection
Bioinformatics techniques- BLAST, KEGG, Phylogenetic analysis, Metagenomics, Whole-genome sequence (NGS) Primer designing
Blotting techniques- SDS page and Western blotting
Analytical techniques- High-Performance Liquid, Fourier Transform Infrared, Spectrophotometer, UV-VIS Spectrophotometer, Fluro Spectrophotometer, NMR, TEM, and SEM
Genetic engineering- Prokaryotic expression system cloning
protein engineering- Purification and production of proteins of interest
Statistical techniques- GraphPad Prism software
Coding basics- C, C, Java, Perl, Python, R, Linux Shell script
1. Shameer Pillarisetti+, Vasukutty Arathy+, Jonghoon Choi, Shin Hyuk Kang, and In-Kyu Park (2023) "CXCR4 Targeting Nanoplatform for Transcriptional Activation of Latent HIV-1 Infected T Cells." ACS Applied Bio Materials, DOI: https://doi.org/10.1021/acsabm.3c00456. [+: Co-first authors]
2. Shameer Pillarisetti, Veena Vijayan, Jayakumar Rangasamy, Rizia Bardhan, Saji Uthaman, and In-Kyu Park (2023) "A Multi-Stimuli Responsive Alginate Nanogel for Anticancer Chemo-Photodynamic Therapy." Journal of Industrial and Engineering Chemistry, 123, 361-370, DOI: https://doi.org/10.1016/j.jiec.2023.03.053.
3. Shameer Pillarisetti+, Saji Uthaman+, Youn-Mook Lim, Jin-Oh Jeong, Rizia Bardhan, Kang Moo Huh, and In-Kyu Park (2023) "Light and immunostimulant mediated in situ re-education of tumor-associated macrophages using photosensitizer conjugated mannan nanoparticles for boosting immuno-photodynamic anti-metastasis therapy." Biomaterials Science 11, 1, 298-306, DOI: https://doi.org/10.1039/D2BM01508K. [+: Co-first authors]
4. Shammer Pillarisetti+, Jagannath Mondal+, Vijayabhaskarreddy Junnuthula, Monochura Saha, Seung Rim Hwang, In-kyu Park, and Yongkyu Lee (2023) "Hybrid exosomes, exosome-like nanovesicles and engineered exosomes for therapeutic applications." Journal of Controlled Release 353, 1127-1149, DOI: https://doi.org/10.1016/j.jconrel.2022.12.027. [+: Co-first authors]
5. Kangmin Noh, Saji Uthaman, Chung-Sung Lee, Yugyeong Kim, Shameer Pillarisetti, Hee Sook Hwang, In-Kyu Park, and Kang Moo Huh (2022) "Tumor intracellular microenvironment-responsive nanoparticles for magnetically targeted chemotherapy." Journal of Industrial and Engineering Chemistry, 111, 121-128, DOI: https://doi.org/10.1016/j.jiec.2022.03.042.
6. Shameer Pillarisetti+, Saji Uthaman+, Kang Moo Huh, Chong-Su Cho, and In-Kyu Park (2021) "Drug-dye-apoptosis inducing micelles for enhancing host immunity against advanced metastatic breast cancer by the combination of low dose chemotherapy and photothermal therapy." Journal of Industrial and Engineering Chemistry 97, 476-484, DOI: https://doi.org/10.1016/j.jiec.2021.03.003. [+: Co-first authors]
7. Saji Uthaman, Shameer Pillarisetti, Hye Suk Hwang, Ansuja Pulickal Mathew, Kang Moo Huh, Joon Haeng Rhee, and In Kyu Park (2021) "Tumor microenvironment regulating immunosenescence independent nanostimulant synergizing with near-infrared light irradiation for antitumor immunity." ACS Applied Materials & Interfaces 13, 4, 4844-4852, DOI: https://doi.org/10.1021/acsami.0c20063
8. Chanuk Jeong, Saji Uthaman, Babak Bagheri, Juwhan Kim, Shameer Pillarisetti, In-Kyu Park, and Yeu-Chun Kim (2021) "Self-assembled heptamethine cyanine dye dimer as a novel theranostic drug delivery carrier for effective image-guided chemo-photothermal cancer therapy." Journal of Controlled Release 329, 50-62, DOI: https://doi.org/10.1016/j.jconrel.2020.11.046.
9. Rimesh Augustine, Saji Uthaman, Nagendra Kalva, Kuen Hee Eom, Kang Moo Huh, Shameer Pillarisetti, In-Kyu Park, and Il Kim (2021) "Two-tailed tadpole-shaped synthetic polymer polypeptide bioconjugate nanomicelles for enhanced chemo-photothermal therapy." Polymer 230, 124061, DOI: https://doi.org/10.1016/j.polymer.2021.124061.
10. Manh-Cuong Vo, Seo-Yeon Ahn, Tan-Huy Chu, Saji Uthaman, Shameer Pillarisetti, Tung Nguyen Thanh Uong, Thangaraj Jaya Lakshmi, Mihee Kim, Ga-Young Song, Sung-Hoon Jung, Deok-Hwan Yang, Jae-Sook Ahn, Hyeoung-Joon Kim, In-Kyu Park and Je-Jung Lee (2021) "A combination of immunoadjuvant nanocomplexes and dendritic cell vaccines in the presence of immune checkpoint blockade for effective cancer immunotherapy." Cellular & Molecular Immunology 18, 6, 1599-1601, DOI: https://doi.org/10.1038/s41423-021-00666-z.
11. Yugyeong Kim, Saji Uthaman, Shameer Pillarisetti, Kangmin Noh, Kang Moo Huh, and In-Kyu Park (2020) "Bioactivatable reactive oxygen speciessensitive nanoparticulate system for chemo-photodynamic therapy." Acta Biomaterialia 108, 273-284, DOI: https://doi.org/10.1016/j.actbio.2020.03.027.
12. Shameer Pillarisetti+, Saji Uthaman+, Kang Moo Huh, Yang Seok Koh, Sangjoon Lee, and In-Kyu Park (2019) "Multimodal composite iron oxide nanoparticles for biomedical applications." Tissue Engineering and Regenerative Medicine 16, 451-465, DOI: https://doi.org/10.1007/s13770-019-00218-7. [+: Co-first authors]
13. Shameer Pillarisetti, S. Maya, S. Sathianarayanan, and R. Jayakumar (2017) "Tunable pH and redox-responsive drug release from curcumin conjugated γ-polyglutamic acid nanoparticles in cancer microenvironment." Colloids and Surfaces B: Biointerfaces 159, 809-819, DOI: 10.1016/j.colsurfb.2017.08.057
Book chapter
1. Shameer Pillarisetti, Veena Vijayan, Saji Uthaman, and In-Kyu Park. "Nanoplatforms for Anticancer Therapy Based on Reactive Oxygen Species." In Science and Applications of Nanoparticles, Jenny Stanford Publishing, pp. 103- 131.